Cloned (Comment) | Organism |
---|---|
coexpression of N-terminally GST-tagged wild-type enzyme with endogenous heme and in complex with its cognate human apocytochrome c and mutants in Escherichia coli | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial membrane | membrane-bound | Homo sapiens | 31966 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
x * 57000, recombinant GST-tagged enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Apocytochrome c + heme | Homo sapiens | - |
Holocytochrome c | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P53701 | - |
- |
Purification (Comment) | Organism |
---|---|
wild-type membrane-bound human GST-tagged enzyme with endogenous heme and in complex with its cognate human apocytochrome c and mutants from Escherichia coli | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Apocytochrome c + heme | - |
Homo sapiens | Holocytochrome c | - |
? | |
Apocytochrome c + heme | conserved His154 is the key ligand to the heme iron, formation of the enzyme-heme complex serves as the platform for interaction with apocytochrome c, the heme is the central molecule mediating contact between enzyme and apocytochrome c. Conserved His19 of the CXXCH motif in apocytochrome c supplies the second axial ligand to heme in the trapped enzyme-heme-cytochrome c complex. Molecular mechanisms, overview | Homo sapiens | Holocytochrome c | - |
? | |
additional information | substrate specificity of the human enzyme, overview. Engineering of a bacterial cytochrome c into a robust substrate for the human enzyme, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57000, recombinant GST-tagged enzyme, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
HCCS | - |
Homo sapiens |
Holocytochrome c synthetase | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | cytochrome c, an essential electron carrier in mitochondria and a critical component of the apoptotic pathway, contains a heme cofactor covalently attached to the protein at a conserved CXXCH motif requiring the mitochondrial protein holocytochrome c synthase | Homo sapiens |